Publications

2017

López-Alonso J.P., Kaminish T., Kikuchi T., Hirata Y., Iturrioz I., Dhimole N., Hase Y., Goto S., Kurita D., Muto A., Zhou S., Naoe C., Takemoto C., Yokoyama S., Mills D.J., Himeno H., Fucini P., and S.R. Connell (2017) RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket, Nucleic Acids Research doi: 10.1093/nar/gkx324.

López-Alonso J.P., Fabbretti A., Kaminishi T., Iturrioz I., Brandi L., Gil-Carton D., Gualerzi C.O., Fucini P., Connell S.R. (2016) Structure of a 30S pre-initiation complex stalled by GE81112 reveals structural parallels in bacterial and eukaryotic protein synthesis initiation pathways. Nucleic Acids Res. 45(4):2179-2187

2016

Heidrich C.G., Mitova S., Schedlbauer A., Connell S.R., Fucini P., Steenbergen J.N., Berens C. (2016) The Novel Aminomethylcycline Omadacycline Has High Specificity for the Primary Tetracycline-Binding Site on the Bacterial Ribosome. Antibiotics 5(4):E32.

Fabbretti A., Schedlbauer A., Brandi L., Kaminishi T., Giuliodori A.M., Garofalo R., Ochoa-Lizarralde B., Takemoto C., Yokoyama S., Connell S.R., Gualerzi C.O., Fucini P (2016) Inhibition of translation initiation complex formation by GE81112 unravels a 16S rRNA structural switch involved in P-site decoding. Proc Natl Acad Sci U S A. 113:E2286-95

2015

Kaminishi T., Schedlbauer A., Fabbretti A., Brandi L., Ochoa-Lizarralde B., He C., Milón P., Connell S.R., Gualerzi C.O., Fucini P. (2015) Crystallographic characterization of the ribosomal binding site and molecular mechanism of action of Hygromycin A.  Nucleic Acids Research 43:10015-25

Schedlbauer A., Kaminishi T., Ochoa-Lizarralde B., Dhimole N., Zhou S., López-Alonso J.P., Connell S.R., Fucini P. (2015) Structural characterization of an alternative mode of tigecycline binding to the bacterial ribosome. Antimicrob Agents Chemother. 59(5):2849-54.

2013

Ciccarelli L., Connell S.R*., Enderle M., Mills D.J., Vonck J., and Grininger M. (2013) Structure and conformational variability of the mycobacterium tuberculosis fatty acid synthase multienzyme complex. Structure 21: 1251–1257.

Gelis I., Vitzthum V., Dhimole N., Caporini M.A,. Schedlbauer A., Carnevale D., Connell S.R., Fucini P., and Bodenhausen G. (2013) Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology. J Biomol NMR. 56: 85-93

2010

Ratje A.H., Loerke J., Mikolajka A., Brünner M., Hildebrand P.W., Starosta A.L., Dönhöfer A., Connell S.R., Fucini P., Mielke T., Whitford P.C., Onuchic J.N., Yu Y., Sanbonmatsu K.Y., Hartmann R.K., Penczek P.A., Wilson D.N., and Spahn C.M.T. (2010) Head swivel on the ribosome facilitates translocation via intra-subunit tRNA hybrid sites. Nature 468:713-716

2009

Schuette J.C., Murphy F.V., Kelley A.C., Weir J.R., Giesebrecht J., Connell S.R., Loerke J., Mielke T., Zhang W., Penczek P.A., Ramakrishnan V., Spahn C.M.T. (2009).  GTPase activation of elongation factor EF-Tu by the ribosome during decoding. EMBO J. 28:755-765

2000-2008

Connell S.R.*, Topf M. Qin Y., Wilson D.N., Mielke T., Fucini P., Nierhaus K.H. and Spahn C.M.T. (2008). A new tRNA intermediate revealed on the ribosome during EF4-mediated back-translocation. NSMB 15:910-915

Wilson D.N., Schluenzen F., Harms J.M.,  Starosta A.L., Connell S.R., and Fucini P. (2008). The oxazolidinone antibiotics perturb the ribosomal peptidyl-transferase center and effect tRNA positioning. PNAS 105:13339-13344

Harms J.M., Wilson D.N., Schluenzen F., Connell S.R., Stachelhaus T., Spahn C.M.T., and Fucini P. (2008). Translational regulation via L11: molecular switches on the ribosome turned on and off by thiostrepton and micrococcin.  Mol. Cell 30:26-38

Connell S.R.*, Takemoto C., Wilson D.N., Wang H., Murayama K., Terada T., Rost M., Schüler M., Giesebrecht J., Dabrowski M., Mielke T., Fucini P., Yokoyama S. and Spahn C.M.T. (2007). Structural Basis for Interaction of the Ribosome with the Switch Regions of GTP-Bound Elongation Factors. Mol. Cell 25:751-764

Schüler M., Connell S.R., Lescoute A., Giesebrecht J., Dabrowski M., Schroeer B., Mielke T., Penczek P.A., Westhof E., Spahn C.M.T. (2006) Structure of the ribosome-bound cricket paralysis virus IRES RNA. NSMB 13:1092-1096

Nonaka L., Connell S.R., and Taylor D.E. (2005). 16S rRNA mutations that confer tetracycline resistance in Helicobacter pylori decrease drug binding in Escherichia coli ribosomes. J Bacteriol. 187(11):3708-12

Gibreel A., Tracz D.M., Nonaka L., Ngo T.M., Connell S.R. and Taylor D.E. (2004). Incidence of antibiotic resistance in Campylobacter jejuni isolated in Alberta, Canada, from 1999 to 2002, with special reference to tet(O)-mediated tetracycline resistance. Antimicrob Agents Chemother. 48(9):3442-50.

Christodoulou J., Larsson G., Fucini P., Connell S.R., Pertinhez T.A., Hanson C.L., Redfield C., Nierhaus K.H., Robinson C.V., Schleucher J., Dobson C.M. (2004). Heteronuclear NMR investigations of dynamic regions of intact Escherichia coli ribosomes. Proc Natl Acad Sci  101(30):10949-54.

Gilbert R.J., Fucini P., Connell S., Fuller S.D., Nierhaus K.H., Robinson C.V., Dobson C.M., and Stuart D.I. (2004). Three-dimensional structures of translating ribosomes by Cryo-EM. Mol Cell. 14(1): 57-66.

Connell S.R., Tracz D.M., Nierhaus K.H. and Taylor D.E. (2003). Ribosomal protection proteins and their mechanism of tetracycline-resistance. Antimicrob. Agents Chemother. 47(12): 3675-3681.

Connell S.R., Trieber C.A., Einfeldt E., Dinos G.P., Taylor D.E., and Nierhaus K.H. (2003). Mechanism of Tet(O)-mediated tetracycline resistance. Embo J. 22(4): 945-953.

Dinos G.P., Connell S.R., Nierhaus K.H., and Kalpaxis D.L. (2003). Erythromycin, roxithromycin, and clarithromycin: Use of slow-binding kinetics to compare their in vitro interaction with a bacterial ribosomal complex active in peptide bond formation. Mol. Pharm 63(3): 617-623.

Connell S.R., Trieber C.A., Stelzl U., Einfeldt E., Taylor D.E., and Nierhaus K.H. (2002). The tetracycline resistance protein, Tet(O), perturbs the conformation of the ribosomal decoding center. Mol Microbiol 45(6): 1463-1472.

Wilson D.N,, Blaha G., Connell S.R., Ivanov P.V., Jenke H., Stelzl U., Teraoka Y., and Nierhaus K.H. (2002). Protein synthesis at atomic resolution: Mechanistics of translation in the light of highly resolved structures for the ribosome. Current Protein and Peptide Science 3: 1-53.

Spahn C.M., Blaha G., Agrawal R.K., Penczek P., Grassucci R.A., Trieber C.A., Connell S.R., Taylor D.E., Nierhaus K.H., and Frank J. (2001). Localization of the ribosomal protection protein Tet(O) on the ribosome and the mechanism of tetracycline resistance. Mol Cell. 7(5): 1037-1045.

Connell S., and Nierhaus K.H. (2000). Translational termination not yet at its end. Chembiochem. 1(4): 250-253.